Romel Menacho‐Melgar

Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in <i>E. coli</i> through Transient Inhibition of Host RecA Activity

Eirik A. Moreb, Benjamin Hoover, Adam Yaseen et al. · ACS Synthetic Biology · 2017 · 61 citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Scalable, two‐stage, autoinduction of recombinant protein expression in <i>E. coli</i> utilizing phosphate depletion

Romel Menacho‐Melgar, Zhixia Ye, Eirik A. Moreb et al. · Biotechnology and Bioengineering · 2020 · 50 citations · Full text

Engineering, Bioengineering, Biotechnology +17

Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin

John S. Decker, Romel Menacho‐Melgar, Michael Lynch · Frontiers in Bioengineering and Biotechnology · 2020 · 41 citations · Full text

Life Sciences, Biotechnology, Microbiology +17

Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in <i>E. coli</i>

Eirik A. Moreb, Zhixia Ye, John P. Efromson et al. · ACS Synthetic Biology · 2020 · 34 citations · Full text

Life Sciences, Bioengineering, Biotechnology +17

Improved two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease

Romel Menacho‐Melgar, Eirik A. Moreb, John P. Efromson et al. · Biotechnology and Bioengineering · 2020 · 32 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +16