Romel Menacho‐Melgar

Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in <i>E. coli</i> through Transient Inhibition of Host RecA Activity

Eirik A. Moreb, Benjamin Hoover, Adam Yaseen et al. · ACS Synthetic Biology · 2017 · 58 citations · Full text

Engineering, Genetics, Molecular Biology +16

Scalable, two‐stage, autoinduction of recombinant protein expression in <i>E. coli</i> utilizing phosphate depletion

Romel Menacho‐Melgar, Zhixia Ye, Eirik A. Moreb et al. · Biotechnology and Bioengineering · 2020 · 48 citations · Full text

Engineering, Molecular Biology, Escherichia Coli +19

Low-Cost, Large-Scale Production of the Anti-viral Lectin Griffithsin

John S. Decker, Romel Menacho‐Melgar, Michael Lynch · Frontiers in Bioengineering and Biotechnology · 2020 · 38 citations · Full text

Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in <i>E. coli</i>

Eirik A. Moreb, Zhixia Ye, John P. Efromson et al. · ACS Synthetic Biology · 2020 · 31 citations · Full text

Improved two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease

Romel Menacho‐Melgar, Eirik A. Moreb, John P. Efromson et al. · Biotechnology and Bioengineering · 2020 · 30 citations · Full text

Engineering, Molecular Biology, Escherichia Coli +20