Fluorescent and bioluminescent genetically encoded Ca²⁺ indicators (GECIs) are an indispensable tool for monitoring Ca²⁺ dynamics in numerous cellular events. Although fluorescent GECIs have a high spatiotemporal resolution, their application is often confined to short-term imaging due to the external illumination that causes phototoxicity and autofluorescence from specimens. Bioluminescent GECIs overcome these pitfalls with enhanced compatibility to optogenetic manipulation and photophysiological processes; however, they are compromised for spatiotemporal resolution. Therefore, there has been a push toward the use of Ca²⁺ indicators that possess the advantages of both fluorescent and bioluminescent GECI for a wide range of applications. To address this, we developed a high-affinity bimodal GECI, GLICO, using a single fluorescent protein-based GECI combined with a split luciferase. Through this novel design, the fusion protein becomes bimodal and possesses Ca²⁺ sensing properties similar to those of its fluorescent ancestor and confers bioluminescence-based Ca²⁺ imaging. GLICO in bioluminescence mode has the highest dynamic range (2200%) of all bioluminescent GECIs. We demonstrated the performance of GLICO in studying cytosolic Ca²⁺ dynamics in different cultured cells in each mode. With the purpose of Ca²⁺ imaging in high Ca²⁺ content organelle, we also created a low-affinity variant, ReBLICO and performed Ca²⁺ imaging of the ER in both fluorescence and bioluminescence modes. The ability to switch between fluorescence and bioluminescence modes with a single indicator would benefit transgenic applications by presenting an opportunity for a wide range of live Ca²⁺ imaging in physiological and pathophysiological conditions.

Israt Farhana

Publications