A newly developed electrochemical biosensor composed of a topological insulator (TI) and metallic DNA (mDNA) is fabricated. The bismuth selenide nanoparticle (Bi₂ Se₃ NP) is synthesized and sandwiched between the gold electrode and another Au-deposited thin layer (Bi₂ Se₃ @Au). Then, eight-silver-ion mediated double-stranded DNA (mDNA) is immobilized onto the substrate (Bi₂ Se₃ @Au-mDNA) for the further detection of hydrogen peroxide. The Bi₂ Se₃ NP acts as the electrochemical-signal booster, while unprecedentedly its encapsulation by the Au thin layer keeps the TI surface states protected, improves its electrochemical-signal stability and provides an excellent platform for the subsequent covalent immobilization of the mDNA through Au-thiol interaction. Electrochemical results show that the fabricated biosensor represents much higher Ag⁺ redox current (≈10 times) than those electrodes prepared without Bi₂ Se₃ @Au. The characterization of the Bi₂ Se₃ @Au-mDNA film is confirmed by atomic force microscopy, scanning tunneling microscopy, and cyclic voltammetry. The proposed biosensor shows a dynamic range of 00.10 × 10^-6 m to 27.30 × 10^-6 m, very low detection limit (10 × 10^-9 m), unique current response (1.6 s), sound H₂ O₂ recovery in serum, and substantial capability to classify two breast cancer subtypes (MCF-7 and MDA-MB-231) based on their difference in the H₂ O₂ generation, offering potential applications in the biomedicine and pharmacology fields.

#	Concept	H-Index	Publications	Citations
1	Engineering	2	2	208
2	Chemistry	2	2	208
3	Nanomaterials	2	2	208
4	Chemical Engineering	1	1	129
5	Materials Science	1	1	79

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