Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies

Carmela Tricarico, Pamela Pinzani, Simonetta Bianchi, +5

Analytical Biochemistry

Polymerase Chain ReactionHuman Tissue BiopsiesNatural SciencesGeneticsMolecular Biology +10

Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase–polymerase chain reaction results and feasibility of molecular analysis by laser microdissection

Pamela Pinzani, Benedetta Salvadori, Lisa Simi, +5

Human Pathology

Breast OncologyOncologyCancer DetectionMedicineCancer Cell Biology +9

Application of a Filtration- and Isolation-by-Size Technique for the Detection of Circulating Tumor Cells in Cutaneous Melanoma

Vincenzo De Giorgi, Pamela Pinzani, Francesca Salvianti, +10

Journal of Investigative Dermatology

EngineeringCirculating Tumor CellsAnalytical MicrosystemsBiomedical EngineeringDermatology +14

Heterogeneity of <i>PIK3CA</i> mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients

Marta Pestrin, Francesca Salvianti, Francesca Galardi, +6

Molecular Oncology

Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer

Francesca De Luca, Giada Rotunno, Francesca Salvianti, +9

Oncotarget

Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows

Rita Lampignano, Martin H. Neumann, Sabrina Weber, +28

Clinical Chemistry

Genetic and epigenetic factors in regulation of microRNA in colorectal cancers

Serena Vinci, Stefania Gelmini, Irene Mancini, +5

Methods

Cancer ResearchColorectal CancersEpigenetic RegulationMedicineGenetics +13

Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification

Stefania Gelmini, Claudio Orlando, Roberta Sestini, +4

Clinical Chemistry

Breast OncologyEngineeringMolecular BiologyNucleic Acid Amplification TestC-erbb-2 Oncogene Amplification +17

Human striatal neuroblasts develop and build a striatal-like structure into the brain of Huntington's disease patients after transplantation

Pasquale Gallina, M. Paganini, Letizia Lombardini, +20

Experimental Neurology

Human Striatal NeuroblastsNeurodegenerative DiseasesDevelopmental BiologyBrain DevelopmentNeuroanatomy +9

Circulating cell-free DNA in plasma of melanoma patients: Qualitative and quantitative considerations

Pamela Pinzani, Francesca Salvianti, Sara Zaccara, +4

Clinica Chimica Acta

MedicineMelanomaBlood CellImmunophenotypingQuantitative Considerations +8