21:I[432972,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 22:T48d,There is strong evidence that the acetylcholine system is responsible for the electric currents which propagate impulses in conducting tissues.^1,2In the eye, high concentrations of acetylcholine have been reported in the corneal epithelium;³cholinesterase has been demonstrated in various other ocular tissues chemically⁴and histochemically;^5,6and high choline acetylase activity has been found in the corneal epithelium.⁷The present study is concerned with determining the concentration and localization of cholinesterase in the cornea.

Methods and Materials

Hestrin's colorimetric technique for determining acetylcholine⁸was used for measurement of cholinesterase activity chemically, and Koelle's method for localization of the cholinesterase in the tissues.^5,6,9-11Normal New Zealand albino rabbits were used throughout.

A. Chemical Determination of Cholinesterase Activity.

—Rabbits were anesthetized with pentobarbital (Nembutal) immediately prior to having the epithelium scraped off their corneas. The fresh epithelium was placed in a small ground glass tissue23:T410,

Introduction

The problem of regeneration and replacement of corneal endothelial cells is still unsolved. It is generally held that there is no evidence of mitosis in the endothelium of adult animals,^1-4although mitoses have been observed in young cats,¹and recently von Sallmann⁵has reported a mitotic index of 2/100,000 in "young adult rabbits." Binder and Binder described only "amitotic" cell division in uninjured rabbit endothelium but both "mitotic and amitotic cell division" in the endothelium 12 hours after injury with a blunt spatula.³Following irrigation of the anterior chamber by α-chymotrypsin, von Sallmann observed greatly increased mitotic activity in some localized areas of Descemet's endothelium. Harding et al. have recently shown that the very weak β-radiation from tritiated thymidine can be detected, by means of autoradiography, in whole-mount preparations of lens epithelium.⁶By this method, incorporation of tritiated thymidine by any cell in the24:T4b7,Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Ophthalmology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Ophthalmology journal

#	Concept	H-Index	Publications	Citations
1	Medicine	4	4	135
2	Anatomy	2	2	44
3	Health Sciences	1	1	57
4	Public Health	1	1	34
5	Biochemistry	1	1	57

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