An Online Four-Dimensional HIC×SEC-IM×MS Methodology for Proof-of-Concept Characterization of Antibody Drug Conjugates

Anthony Ehkirch, Valentina D’Atri, Florent Rouvière, +8

Analytical Chemistry

EngineeringQuantitative PharmacologyBiomedical EngineeringNative MsBioanalysis +18

Hyphenation of size exclusion chromatography to native ion mobility mass spectrometry for the analytical characterization of therapeutic antibodies and related products

Anthony Ehkirch, Oscar Hernandez‐Alba, Olivier Colas, +3

Journal of Chromatography B

ImmunologyIon Mobility SpectrometrySize Exclusion ChromatographyBioanalysisImmunochemistry +14

A Novel Online Four-Dimensional SEC×SEC-IM×MS Methodology for Characterization of Monoclonal Antibody Size Variants

Anthony Ehkirch, Alexandre Goyon, Oscar Hernandez‐Alba, +9

Analytical Chemistry

EngineeringImmunologyBiological Mass SpectrometryImmunophenotypingImmunotherapeutics +20

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

Thomas Botzanowski, Stéphane Erb, Oscar Hernandez‐Alba, +7

mAbs

Drug TargetBiological Mass SpectrometryMedicinal ChemistryMiddle- Level CharacterizationThiol Groups +19

Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies

Oscar Hernandez‐Alba, Elsa Wagner‐Rousset, Alain Beck, +1

Analytical Chemistry

Immunocytochemical TechniqueImmunologyMolecular BiologyCollision-induced UnfoldingProtein Folding +15

Pushing the limits of native MS: Online SEC-native MS for structural biology applications

Evolène Deslignière, Marie Ley, Maxime Bourguet, +5

International Journal of Mass Spectrometry

Structural BioinformaticsBiomolecular ToolMedicineNative MsNatural Sciences +9

A Case Study to Identify the Drug Conjugation Site of a Site-Specific Antibody-Drug-Conjugate Using Middle-Down Mass Spectrometry

Oscar Hernandez‐Alba, Stéphane Houel, Steve Hessmann, +7

Journal of the American Society for Mass Spectrometry

Middle-down Mass SpectrometryMolecular BiologyMedicinal ChemistryBioanalysisImmunochemistry +19

Toward Automation of Collision-Induced Unfolding Experiments through Online Size Exclusion Chromatography Coupled to Native Mass Spectrometry

Evolène Deslignière, Anthony Ehkirch, Thomas Botzanowski, +3

Analytical Chemistry

State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

Evolène Deslignière, Anthony Ehkirch, Bastiaan L. Duivelshof, +7

Pharmaceuticals

GlycobiologyBiological Mass SpectrometryImmunotherapeuticsIon Mobility SpectrometryMedicinal Chemistry +22

High-Resolution IMS–MS to Assign Additional Disulfide Bridge Pairing in Complementarity-Determining Regions of an IgG4 Monoclonal Antibody

Evolène Deslignière, Thomas Botzanowski, Hélène Diemer, +8

Journal of the American Society for Mass Spectrometry

Disulfide PeptidesImmunocytochemical TechniqueImmunologyGlycobiologyImmunotherapy +19