Knowledge about the primary factor controlling stable isotope ratios of particulate nitrogen (δ¹⁵N_PN) and total dissolved nitrogen (δ¹⁵N_TDN) in a subtropical reservoir can improve the understanding of regional and global nitrogen cycles. Taking Lianhe Reservoir as a representative subtropical reservoir, we studied the spatial and temporal distributions of δ¹⁵N_PN andδ¹⁵N_TDN and their relationships with the surrounding physicochemical factors and phytoplankton. The results showed that variations in δ¹⁵N_PN and δ¹⁵N_TDN followed seasonal thermal cycles. The values of δ¹⁵N_TDN were inversely proportional to those of δ¹⁵N_PN. PCA showed that phytoplankton cell density and pH were the primary drivers of the variation of δ¹⁵N_PN (45.2%). The primary factors influencing δ¹⁵N_TDN were Chl a and phytoplankton cell density, which both indicated phytoplankton biomass. We also determined that the dominant species was Microcystis densa during the thermal stratification period and Staurodesmus aristiferus during the mixing period. Laboratory experiments showed that δ¹⁵N_PN values in both M. densa (from 19.5 to 14.6‰) and S. aristiferus (from 19.4 to 16.0 ‰) media decreased significantly as the algal cells grew. Furthermore, the δ¹⁵N_TDN values increased from 4.9 to 7.9‰ and from 4.7 to 6.9‰ in M. densa and S. aristiferus media, respectively, when the δ¹⁵N_PN values decreased. These experimental results were consistent with field investigation results and indicated that variations in δ¹⁵N_PN and δ¹⁵N_TDN were mainly controlled by phytoplankton cell density, especially the cell density of the dominant species, in both the thermal stratification and mixing periods. The results also suggested that cell density, not phytoplankton species, was the key factor regulating the distribution of nitrogen stable isotopes. These results together indicated that phytoplankton cell density is the primary factor in the regulation of nitrogen stable isotope composition and that its influence is greater than that of other physical and chemical factors. This study provided detailed information supporting the primary role of phytoplankton in the nitrogen geochemical cycle and improved the understanding of biochemical processes in natural subtropical reservoirs.

#	Concept	H-Index	Publications	Citations
1	Engineering	1	1	13
2	Medicine	1	1	25
3	Biology	1	1	25
4	Microbiology	1	1	25
5	Climate Change	1	1	13

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