American Journal of Veterinary Research · 1989 · 76 citations · 0 references
Veterinary MedicineInfectious DiseasesVeterinary ParasitologyDiagnostic ParasitologyImmunology Of Infectious DiseasesVeterinary Parasitic DiseasesAnimal Infectious DiseasesAntigen Detection AssaysParasitic Disease DiagnosisSerological DiagnosisVeterinary Diagnostic MicrobiologyVeterinary ImmunologyAntibody Titer AnalysisCapture Antibody AssaysExperimental Host InfectionExperimental Infection AssaysSoft Tissue CystsToxoplasma DetectionToxoplasma Gondii Detection
Subclinical Toxoplasma gondii infection was induced in young and adult cats by oral administration of tissue cysts. An antibody-capture ELISA to detect anti-Toxoplasma IgM-class antibodies in the serum of cats was developed. The serologic response to experimental infection was followed in the 2 groups of cats by use of anti-Toxoplasma IgM and IgG detection. This study shows that anti-Toxoplasma IgM-class antibody titers develop early in the course of experimental infection in cats and that the combination of IgM- and IgG-class antibody titer measurement can aid in the detection of recent subclinical toxoplasmosis.