Early expression of a collagenase‐like hatching enzyme gene in the sea urchin embryo.

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

Andrew P. Feinberg, Bert Vogelstein · Analytical Biochemistry · 1983 · 25.2K citations

Life Sciences, Genetics, Molecular Biology +7

Improved tools for biological sequence comparison.

William R. Pearson, David J. Lipman · Proceedings of the National Academy of Sciences · 1988 · 11.3K citations · Full text

Life Sciences, Bioinformatics, Biology +15

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Haim Aviv, Philip Leder · Proceedings of the National Academy of Sciences · 1972 · 6.9K citations

Life Sciences, Biochemistry, Molecular Biology +10

A simple and very efficient method for generating cDNA libraries

Ueli Gubler, Beth J. Hoffman · Gene · 1983 · 4.6K citations

Life Sciences, Molecular Biology, Molecular Genetics +10