BioTechniques · 2000 · 352 citations · 12 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationGenetic EngineeringGene Expression RegulationPromoter EngineeringTransgene Expression ControlTransgenesis StrategiesStable Gene TransferTransfection EfficiencyTransfection ReagentsTransformation Vector BackbonesTransgenic DrosophilaTransient Gene Transferβ-Galactosidase Reporters
the efficiency of transfection. In light of the fact that ECs are difficult to transfect, the success of this method is warranted for a wider range of cells with different origins. In fact, we have obtained similar results in temporally transfecting NIH 3T3 cells using this method (data not shown). In addition, this method of temporal transfection might have utility in reversing cellular abnormalities through genetic intervention of the temporally introduced gene.
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