Growth of myxamoebae of the cellular slime mould <i>Dictyostelium discoideum</i> in axenic culture

TLDR

The study presents a simple axenic medium for growing Dictyostelium discoideum myxamoebae. The authors describe the medium and procedures for cultivating the myxamoebae in liquid and solid media, and the conditions to trigger their differentiation. Other items 1, 2, and 3.

Abstract

1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.