In vitro biofilm activity of Candida species isolated from Anatolian buffaloes with mastitis in Western Turkey.

Abstract

In the present study, identification of 66 Candida isolates obtained from mastitic Anatolian buffalo quarter milk samples was achieved using the API 20 C AUX system. Among the isolates, the most common species were Candida krusei (27.3%), followed by Candida rugosa (16.7%), Candida kefyr (12.1%) and Candida tropicalis (10.6%). The other species were detected as Candida albicans (9.1%), Candida zeylanoides (6.1%), Candida parapsilosis (6.1%), Candida guilliermondii (4.5%), Candida famata (3.0%), Candida glabrata (3.0%) and Candida ciferrii (1.5%). A total of 53 (80.3%) Candida isolates produced the biofilm. All C. krusei, C. tropicalis, C. parapsilosis and C. guilliermondii strains were determined as biofilm positive. However, C. ciferrii did not produce any biofilm. This is the first study to investigate in vitro biofilm activity of different Candida species isolated from Anatolian buffalo mastitic milk in Turkey. We consider that this study may be a helpful tool for future research into mastitis caused by Candida species. Key words: biofilm activity, buffalo, Candida, mastitis