HPLC enantiomeric resolution of nebivolol on normal and reversed amylose based chiral phases.

Abstract

Racemic nebivolol, a beta-adrenergic blocker showing very promising beta-adrenergic antagonist properties in comparison to other beta-adrenergic blockers has been resolved by HPLC under normal and reversed phase modes. The columns used were Chiralpak AD and Chiralpak AD-RH containing amylose tris (3,5-dimethyl phenyl carbamate) as the chiral selector. The mobile phases used were pure ethanol and 1-propanol. The flow rates used were 0.5, 1.0 and 1.5 ml/min. The best resolution was achieved at 0.5 ml/min. flow rate with ethanol and 1-propanol on both Chiralpak AD and Chiralpak AD-RH stationary phases. The values of infinity for both alcohols on Chiralpak AD were 1.38 while on Chiralpak AD-RH these values were 1.41 and 1.38 respectively. The values of Rs for ethanol and 1-propanol were 2.63 and 1.71 on Chiralpak AD and 1.73 and 1.76 on Chiralpak AD-RH respectively.