A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells.

A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

Life Sciences, Microbiology, Molecular Biology +13

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Peter J. Southern, Paul Berg · PubMed · 1982 · 2.8K citations

Life Sciences, Genetics, Molecular Biology +17

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

Manuel Dagert, S. Dusko Ehrlich · Gene · 1979 · 1.5K citations

Life Sciences, Microbiology, Bacteriology +3

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates

Robert F. Weaver, Charles Weissmann · Nucleic Acids Research · 1979 · 1.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +5

Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor

Michael Wigler, Àngel Pellicer, Saul J. Silverstein et al. · Cell · 1978 · 1.3K citations

Life Sciences, Genetics, Molecular Genetics +7