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Effect of Metal Ions on the Degradation and Adsorption of Two Cellobiohydrolases on Microcrystalline Cellulose
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2001
Year
Chemical EngineeringMetal IonsBa ++Adsorption ExperimentsBiochemistryMicrocrystalline CelluloseAdsorption AffinityEngineeringBioremediationBiochemical EngineeringBiotechnologyChemisorptionBioelectrochemical ReactorAdsorptionEnzyme ImmobilizationHemicelluloseNanocelluloseWood Component
Ba ++ , Pb ++ , and Cd ++ . The addition of Mn ++ , Ba ++ , and Ca ++ (10 mM) during the hydrolysis of Avicel PH 101 caused an increase in the total reducing sugar (TRS) for CBH I by 142, 135, and 114 percent, respectively. Those for CBH II increased by 177, 175, and 115 percent, respectively. The Mn ++ was the most stimulatory metal ion, whereas Hg ++ was the most inhibitory metal ion. The adsorption experiments were performed to investigate how the influence of Mn ++ and Hg ++ on the hydrolysis is related to the adsorption of cellobiohydrolases on cellulose. The increase in TRS during hydrolysis by adding Mn ++ caused an increase in adsorption affinity ( Kad) and tightness (ΔHa). While, the decrease of TRS during hydrolysis by adding Hg ++ caused a decrease in the adsorption affinity ( Kad) and tightness (ΔHa). These results indicate the changes in the tightness and affinity of adsorption by adding metal ions play a crucial role in the degradation of the microcrystalline cellulose.