Potyvirus associated with mosaic disease on patchouli (Pogostemon cablin (Blanco) Benth.) plants in Indonesia.

Abstract

This study was carried out to identify the virus associated with mosaic disease on patchouli in Indonesia. The diseased plant samples collected from Garut, Ciamis, Bogor (West Java) and West Pasaman (West Sumatera) strongly reacted with Potyvirus antiserum by enzyme-linked immunosorbent assay, but did not show any signal with antisera to Cucumber mosaic virus, Tobacco mosaic virus, Broad bean wilt virus 1 and Broad bean wilt virus 2; except the samples collected from Brebes (Central Java) which strongly reacted with Broad bean wilt virus 2 (Fabavirus) only. Besides that, the diseased plant samples collected from North Sumatera (Pakpak Bharat) did not react to any antiserum tested. The Potyvirus was then isolated. Positive results were obtained using a reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect and identify Potyvirus from nucleic acid extracts of the symptomatic patchouli plants, using a pair of degenerate primers specific for Potyvirus CP gene. The sequence of this RT-PCR fragment, consisted of 800 bp, confirmed association of a Potyvirus with mosaic disease on patchouli plants in Indonesia. Potyvirus infecting patchouli plants in Indonesia are closely related to Telosma mosaic virus (TeMV) and Passionfruit woodiness virus (PaWP). This paper is the first report that TeMV, PaWP and Fabavirus are associated with mosaic disease on patchouli plants in Indonesia.