Military Medicine · 2003 · 12 citations · 0 references
Life SciencesMicrobiologyMolecular BiologyBacteriologyDiagnostic MicrobiologyMolecular DiagnosticsInfectious Disease DetectionInfectious Disease Molecular TestingMolecular DiagnosisPathogen DetectionPolymerase Chain ReactionRespiratory Pathogen DiagnosticsMolecular Detection AssaysMolecular Detection Of BacteriaMolecular Pathogen DetectionPathogen Dna DetectionPathogen Nucleic Acid DetectionPolymerase Chain Reaction (Pcr) AssaysPolymerase Chain Reaction (Pcr)-based IdentificationPolymerase Chain Reaction Detection
Real-time fluorescence polymerase chain reaction is a microbial identification method that can provide rapid and accurate results using a field-deployable thermocycler, the RAPID ("ruggedized" advanced pathogen identification device). A Yersinia pestis-specific TaqMan assay required approximately 75 minutes and achieved a sensitivity of 100 fg of Y. pestis genomic DNA (20 genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial species encountered in the respiratory tract resulted in no false positives. No cross-reaction occurred with human genomic DNA.