A Comparison of Reduced Nicotinamide Adenine Dinucleotide-Cytochrome c Reductases from Heart Mitochondria and Their Formation from the Respiratory Chain-linked Dehydrogenase

Abstract

I O'* exchanges during myosin-catalyzed hydrolysis Conditions--O.1 M KCl; 0.125 M Tris, pH 7.4; 0.01 M MgClz; 6 mg of myosin per ml; 25".Initial atom y. of Pi in "P" experiments was approximately 3.0.Atom 70 excess of water in "W" experiments was about 1.4.Calculations-Where: Xi = atom 70 excess Or8 of Compound i, Mi = molarity of compound i, and PO = initial Pi, Ph = Pi produced by hydrolysis, Pt = total inorganic phosphate, and w = water.Column 8 = 1 -(Xp,Mp,/X,,M,,).