Bio-Medical Materials and Engineering · 2006 · 19 citations · 8 references
EngineeringBioengineeringCell BiologyCell ProliferationRegenerative MedicineTissue EngineeringAnimal Cell CultureCell CultureCell Culture TechnologyCell Growth KineticsGrowth Factor SignalingMammalian Cell CultureCell Culture ConditionsCell Culture Media FormulationCulture Medium FormulationCulture Medium OptimizationGrowth RatesKeratinocyte CultureNutrient Medium Optimization
UNLABELLED: Our objective was to formulate a medium for monolayer culture optimising both keratocyte growth and preservation of the keratocyte phenotype. METHODS: An experimental matrix selected 14 media to test, using 7 components. Selection criteria were growth rates over 5 passages and expression of the CD34 marker. RESULTS: Acetylcholine, insulin and vitamin C had no effect on growth and differentiation. The DMEM + Ham F12 1 : 1 based medium was selected for its initial effect on growth. At concentrations of 5 ng/ml, b-FGF improved the percentage of CD34+ cells without reducing growth rates. New-born calf serum (NCS) had a greater effect on growth than foetal calf serum (FCS). We showed three major interactions: between b-FGF and IGF-1, FCS and IGF-1 and NCS and b-FGF. CONCLUSION: We selected the following medium, which provides optimal growth and preservation of the CD34+ phenotype: DMEM/HAM-F12 + 10% NCS + 5 ng/ml b-FGF + antibiotics.
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Functional Human Corneal Equivalents Constructed from Cell Lines
May Griffith, Rosemarie Osborne, Réjean Munger et al. · Science · 1999 · 463 citations