Attenuation of diabetic disorders in experimentally induced diabetic rat by methanol extract of seed of Holarrhena antidysenterica.

Abstract

The study was undertaken to evaluate the therapeutic efficacy of methanol extract of seed of Holarrhena antidysenterica in streptozotocin (STZ) induced diabetic rats. Extract was administered orally at a dose of 400 mg / kg body weight / day for 21 days, to STZ-induced diabetic rats. The fasting blood glucose level was decreased significantly after the treatment of methanolic extract to diabetic rats. Activities of hexokinase and glucose-6-phosphate dehydrogenase in liver as well as glycogen levels in liver and skeletal muscle all were increased significantly in extract treated diabetic group in compare with untreated diabetic control group. Activities of hepatic glucose-6-phosphatase were recovered significantly in extract treated diabetic group in respect to untreated diabetic group. To assess the antihyperlipidemic activities of this extract, we have also measured the serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLc), very low density lipoprotein cholesterol (VLDLc) and high density lipoprotein cholesterol (HDLc). The said extract also resulted a significant recovery in above mentioned biomarkers of lipid profile when treated to STZ-induced diabetic rat. Resettlement of all these parameters after the treatment of methanol extract of seed of H. antidysenterica is promising which has been reflected here from the comparison with the antidiabetic drug i.e. glibenclamide. After the monitoring of GOT and GPT activities in serum, it has been noted that the extract significantly correct the activities of these enzymes in STZ-induced diabetic rat. The result of this experiment demonstrated that the extracts of the said plant part possess a promising antidiabetic efficacy.