REVERSAL OF AUREOMYCIN INHIBITION OF BACTERIAL CELL-FREE NITRO REDUCTASE BY MANGANESE

Abstract

SUMMARY 1. DPNH can replace DPN and I-malate in the reduction of chloram- phenicol to the corresponding arylamine mediated by cell-free extracts of Escherichia coli. 2. When DPNH replaces DPN and I-malate, Aureomycin no longer is inhibitory and DPNH can reverse the inhibitory activity of Aureomycin in the present system. 3. Mn++ is necessary for the formation of both DPNH and arylamine and added Mntt- reverses the inhibition of arylamine formation from chlor- amphenicol. 4. The implications of these findings with respect to the antibiotic ac- tivity of Aureomycin are discussed. BIBLIOGRAPHY 1. Saz, A. K., and Marmur, J., Proc. Sot. Exp. Biol. and Med., 82,783 (1953). 2. Sas, A. K., and Slie, R. B., Arch. Biochem. and Biophys, in press. 3. Van Meter, J. C., and Oleson, J. J., Science, 113, 273 (1951). 4. Loomis, W. F., Science, 111, 474 (1950). 5. Van Meter, J. C., Spector, A., Oleson, J. J., and Williams, J. H., Proc. Sot. Ezp. Biol. and Med., 81, 215 (1952). 6. Albert, A., Nature, 172, 201 (1953). 7. Soncin, E., Arch. internat. pharmacod. et tht%ap., 94, 346 (1953). 8. Meister, A., Sober, H. A., and Peterson, E. A., J. Biol. Chem., 208, 89 (1954).