In Vitro Propagation of Japanese Plum (Prunus salicina Lindl. cv. Calita)1

Abstract

Abstract Tissue cultures of ‘Calita’ Japanese plum started from 5 to 7 mm long shoot tips proliferated at a rate of 10:1 to 20:1 per month when grown on a modified Murashige and Skoog medium with 3% sucrose, 0.75% agar and (in mg/liter) 0.4 thiamine HCl, 100 myo-inositol, 1.0 6-benzylamino purine (BA), 0.1 gibberellic acid (GA3) and 0.1 indolebutyric acid (IBA). Best rooting results were obtained with 2 or 4 mg/liter indolebutyric acid (IBA) at 21°C; raising the temperature to 26° or 30° slowed and decreased rooting unless 0.1 mg/liter GA 3 was included in the medium. Activated charcoal in the medium drastically reduced rooting. Rooted cuttings were easily established in soil.