PubMed Central · 1950 · 19 citations · 0 references
MedicineCell BiologyPathologyHistologyHistopathologyMicroscopic AnatomyAntigen Detection AssaysFluorescence ImagingFluorescence MicroscopyImmunohistochemistryLight MicroscopyMicroscopic ExaminationAntigen-antibody InteractionsFluorescent StainingHistochemical MicroscopyImmunofluorescence AssaysImmunofluorescence StainingImmunohistochemical DetectionImmunohistochemical StainingImmunohistochemistry Assays
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.