Restriction endonuclease cleavage at the termini of PCR products.

Abstract

We tested 20 commonly used restriction endonucleases for their ability to recognize and digest sequences at the extreme ends of DNA fragments. For restriction enzymes which are unable to digest a site located near an end, we describe a simple protocol for self-ligation of the amplification product prior to digestion to allow cleavage of the site and subsequent subcloning