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First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex

Godwin Kwakye-Nuako, Mba-Tihssommah Mosore, Christopher Duplessis, Michelle Bates, Naiki Puplampu, Israel Mensah-Attipoe, Kwame Desewu, Godwin Afegbe, Richard Harry Asmah, Manal B. Jamjoom,

International Journal for Parasitology · 2015 · 80 citations · 21 references

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Abstract

An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.

References

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Persistence of<i>Leishmania</i>Parasites in Scars after Clinical Cure of American Cutaneous Leishmaniasis: Is There a Sterile Cure?

Mitzi G. Mendonça, Maria Edileuza Felinto de Brito, Eduardo Henrique Gomes Rodrigues et al. · The Journal of Infectious Diseases · 2004 · 183 citations · Full text

Evolution of the genus Leishmania revealed by comparison of DNA and RNA polymerase gene sequences1Note: Nucleotide sequence data reported in this paper have been submitted to the GenBank™ data base with the accession numbers: POLA/RPOIILS (AF009134/AF009153, Leishmania adleri); (AF009135/NS, Leishmania aethiopica); (AF009136/AF009154, Leishmania amazonensis); (AF009137/NS, Leishmania arabica); (AF009138/AF009155, Leishmania braziliensis); (AF009139/NS, Leishmania chagasi); (AF009140/AF009156, Leishmania deanei); (AF009141/AF009157, Leishmania donovani); (AF009142/AF009158, Endotrypanum monterogeii); (AF009143/AF009159, Leishmania gymnodactyli); (AF009144/AF009160, Leishmania herreri); (AF009145/AF009161, Leishmania hertigi); (AF009146/AF009162, Leishmania hoogstraali); (AF009147/NS, Leishmania infantum); (AF009148/AF009163, Leishmania major); (AF009149/AF009164, Leishmania mexicana); (AF009150/AF009165, Leishmania panamensis); (AF009151/AF009166, Leishmania tarentolae); (AF009152/AF009167, Leishmania tropica).1

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