The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

Haider Raza, Annie John, Abderrahim Nemmar · Cellular Physiology and Biochemistry · 2013 · 47.3K citations · Full text

Life Sciences, Immunology, Toxicology +17

High efficiency transformation of E.coli by high voltage electroporation

William J. Dower, Jeff F. Miller, Clifton W. Ragsdale · Nucleic Acids Research · 1988 · 2.8K citations · Full text

Life Sciences, Biotechnology, Microbiology +13

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

Cathie T. Chung, Sanna‐Mari Niemelä, R.H. Miller · Proceedings of the National Academy of Sciences · 1989 · 1.6K citations · Full text

Life Sciences, Biotechnology, Microbiology +9