Methods in molecular biology · 2013 · 17 citations · 7 references
EngineeringCancer BiologyCell BiologyBiomedical ImagingBiosensorsMolecular ImagingBiological SensorsCell-based BiosensorsMolecular Imaging In OncologyTumor ImagingWhole-cell BiosensorsBiosensor ImagingLive-cell Fluorescence ImagingLive-cell ImagingLive-cell Imaging ApplicationsLive-cell Imaging ProbesLiving Cell SensorsMammalian Cell Biosensors
Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.
7
Spatiotemporal dynamics of RhoA activity in migrating cells
Olivier Pertz, Louis Hodgson, Richard Klemke et al. · Nature · 2006 · 826 citations