Introduction to cell and tissue culture: theory and technique

Abstract

1.Introduction. 2.Setting up a Cell Culture Lab. 3.The Physical Environment. 4.Media. 5.Standard Cell Culture Techniques. 6.Looking at Cells. 7.Contamination, How to Avoid It, Recognize It, and Get Rid of It When It's There. 8.Special Considerations for Serum Free Culture-Established Cell Lines. 9.Primary Cultures. 10.Establishing a Cell Line. 11.Special Growth Conditions. 12.Cell Culture for Commercial Settings. Glossary. Appendices: Equipment List. List of Cell Lines and Where to Get Them. Preparation of Bovine Pituitary Extract. Index.