High Performance Liquid Chromatographic Analysis of Heroin by Reverse Phase Ion-Pair Chromatography

Abstract

Abstract A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, phosphoric acid, and methane sulfonic acid mobile phase and a microparticulate octadecylsilane bonded column has been developed for the rapid, sensitive, selective, and accurate analysis of heroin. Retention data relative to heroin and 220 : 254 nm absorbance ratios are presented for the various adulterants in heroin exhibits.