PubMed · 1999 · 72 citations · 0 references
Veterinary MedicineGeneticsInfectious DiseasesDiagnostic MicrobiologyDiagnostic ParasitologyParasitologyHelminth DiagnosticsLarval Stage IdentificationMolecular Diagnosis Of ParasitesParasitic Disease DiagnosisParasitic Infestation DetectionAnisakis SpeciesExact IdentificationLarval Species IdentificationMolecular Species IdentificationParasite Species IdentificationPolymerase Chain Reaction-restriction AnalysisRestriction Fragment Length Polymorphism AnalysisSpecies-specific Identification
A set of genetic markers, based on PCR-RFLPs of three diagnostic restriction enzymes (Hhal, Hinfl and Taql), which proved to be suitable for the identification of the species of the genus Anisakis, was used for the first molecular identification of a larva obtained by endoscopy in a case of gastric anisakidosis, in a 51 year old woman from Southern Italy. The analysis of the restriction profiles obtained allowed the larva to be identified as Anisakis pegreffii, one of the three sibling species of the A. simplex complex. PCR-RFLP proved to be a cost-effective and reliable tool for the exact identification of Anisakis larvae recovered from infected humans.