International Journal of Agriculture Sciences · 2011 · 10 citations · 0 references
AgriculturePlant BiologyPlant SciencesPlant BiochemistryPlant BiotechnologyPlant PropagationMedicinal Plant ProductionPlant Growth-promoting MicroorganismsRoot ColonizationRoot DevelopmentCultivation Of Medicinal PlantsHairy Root CultureLarge-scale ProductionProduction ScaleRoot Transformation
An experiment was conducted for induction & establishment of Agrobacterium mediated hairy root culture of Coleus forskohlii was developed & co-cultivation on hormone free semisolid MS Medium with B5vitamins.Sterilization period was standardized for the stem & leaf explant using 0.1% bavistin and 0.1% Hgcl2 with varied intervals It resulted in the emergence of hairy roots from the leaf explant , stem explant after 20th day of infection .The transformation of hairy roots was established and confirmation of forskolin content was done by HPLC. Selection of high forskolin can provide an alternative source of large scale production of forskolin. Keywords- Agrobacterium rhizogene , Coleus forskohlii ,Hairy root, HPLC