Survival advantage of native and engineered T cells is acquired by mitochondrial transfer from mesenchymal stem cells

Abstract

Artificial MitoT prevents STS-induced apoptosis of human CD3⁺ T cells by interfering with the caspase pathway. Furthermore, we observed that MitoT confers protection to apoptosis induced by electroporation in MitoT^pos CAR T-engineered cells, potentially improving their metabolic fitness and resistance to environmental stress. These results widen the physiological perspective of organelle-based therapies in immune conditions while offering potential avenues to enhance CAR-T treatment outcomes where their viability is compromised.