A dual-channel sensing platform for ATP and copper ions via the target-induced peroxidase mimics activity and fluorescence variations of iron oxide nanoparticles-modified metal-organic frameworks

Abstract

The simultaneous or continuous detection of multiple targets in complex samples is for various applications, notably in medical diagnostics and environmental monitoring. A novel dual-channel colorimetric and fluorescent method has been developed for the continuous detection of adenosine triphosphate (ATP) and copper ions based on PCN-224@Fe 2 O 3 , which exhibits both fluorescent and enhanced peroxidase-like (POD-like) properties. The synthesis of PCN-224@Fe 2 O 3 involved the in-situ growth of ferric oxide on Zr-based metal-organic frameworks (PCN-224). Through catalyzing the colorless compound 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) in the presence of H 2 O 2 , PCN-224@Fe 2 O 3 allows for the detection of ATP and copper ions. The unique mechanism involves ATP weakening the POD-like activity of PCN-224@Fe 2 O 3 by forming Zr-O-P bonds with Zr in PCN-224, which also leads to fluorescence recovery. Copper ions, through chelating with ATP, counteract the effect of ATP on PCN-224@Fe 2 O 3 , resulting in increased POD-like activity and the amount of oxTMB alongside the quenching of the fluorescence of the system. This facilitates colorimetric “on-off-on” and fluorescence “off-on-off” switch sensing for ATP and Cu 2+ . The application of PCN-224@Fe 2 O 3 in the detection of ATP and Cu 2+ in human serum yielded satisfactory results. This innovative approach not only provides a means to regulate the mimic activity of oxides but also presents a convenient, sensitive, and accurate detection method for ATP and Cu 2+ , significantly broadening the potential application of MOFs and nanozymes in bioanalysis. • A dual-channel sensing platform for the continuous detection of ATP and Cu 2+ was developed. • PCN-224@Fe 2 O 3 possesses fluorescent properties and enhanced POD-like activity. • Target-induced mimics activity and fluorescence variations promise sensitive and selective detection. • The detection mechanisms were investigated in detail by experimental analysis. • Continuous detection of ATP and Cu 2+ by the dual-channel sensing platform with good performance.