<i>Methylomonadaceae</i> was the active and dominant methanotroph in Tibet lake sediments

Abstract

Methane (CH₄), an important greenhouse gas, significantly impacts the local and global climate. Our study focused on the composition and activity of methanotrophs residing in the lakes on the Tibetan Plateau, a hotspot for climate change research. Based on the field survey, the family <i>Methylomonadaceae</i> had a much higher relative abundance in freshwater lakes than in brackish and saline lakes, accounting for ~92% of total aerobic methanotrophs. Using the microcosm sediment incubation with ¹³CH₄ followed by high throughput sequencing and metagenomic analysis, we further demonstrated that the family <i>Methylomonadaceae</i> was actively oxidizing CH₄. Moreover, various methylotrophs, such as the genera <i>Methylotenera</i> and <i>Methylophilus</i>, were detected in the ¹³C-labeled DNAs, which suggested their participation in CH₄-carbon sequential assimilation. The presence of CH₄ metabolism, such as the tetrahydromethanopterin and the ribulose monophosphate pathways, was identified in the metagenome-assembled genomes of the family <i>Methylomonadaceae</i>. Furthermore, they had the potential to adapt to oxygen-deficient conditions and utilize multiple electron acceptors, such as metal oxides (Fe³⁺), nitrate, and nitrite, for survival in the Tibet lakes. Our findings highlighted the predominance of <i>Methylomonadaceae</i> and the associated microbes as active CH₄ consumers, potentially regulating the CH₄ emissions in the Tibet freshwater lakes. These insights contributed to understanding the plateau carbon cycle and emphasized the significance of methanotrophs in mitigating climate change.