A comprehensive analysis of antibiotic resistance genes in the giant panda gut

Abstract

In this study, we have successfully constructed a comprehensive database of metagenome-assembled genomes (MAGs) pertaining to the gut microbiota of the giant panda. Through our analysis, we have identified significant reservoirs of antibiotic resistance genes (ARGs), namely <i>Escherichia coli, Citrobacter portucalensis</i>, and <i>Klebsiella pneumoniae</i>. Furthermore, we have elucidated the primary contributors to ARGs, including <i>Streptococcus alactolyticus</i> and <i>Clostridium SGBP116</i>, in both captive and wild pandas. Additionally, our findings have demonstrated a higher prevalence of ARGs in the metagenome, with notable expression of the <i>RPOB2</i> gene in <i>S. alactolyticus</i>. Crucially, 1217 ARGs shared homology with human gut ARGs, underscoring the interaction relationship between pandas and human microbiomes. These findings are instrumental in understanding the antibiotic resistance landscape in the giant panda's gut, providing a framework for developing strategies to combat antibiotic resistance and safeguard the health of this endangered species.