1e:T4e8,Microplastics (size of plastic debris <5 mm) occur in various environments worldwide these days and cause detrimental effects on biota. However, the behavioral responses of fish to microplastics in feeding processes are not well understood. In the present study, juveniles from four fish species and two common shapes of microplastics were used to explore fish feeding responses. We found swallowing-feeding fish ingested more pellets than filtering- and sucking-feeding fish. With high-definition and high-speed observational experiments, we found that all species did not actively capture microfibers; instead, they passively sucked in microfibers while breathing. Surprisingly, fish showed a rejective behavior, which was spontaneously coughing up microfibers mixed with mucus. Nevertheless, some of the microfibers were still found in the gastrointestinal tracts and gills of fish, while abundances of ingested microfibers were increased in the presence of food. Our findings reveal a common phenomenon that fish ingest microplastics inadvertently rather than intentionally. We also provide insights into the pathways via which microplastics enter fish and potential strategies to assess future ecological risk and food safety related to microplastics.1f:T537,Although microplastic (MP) pollution in freshwater systems is gaining attention, our knowledge of its distribution in small waterbodies is scarce. Small waterbodies are freshwater habitats to many species, including amphibians, that are vulnerable to MP pollution. This study analyzed the distribution and characteristics of MPs in 25 small waterbodies from the Yangtze River Delta, China. MPs were detected in surface water, sediment, and tadpoles with abundances ranging from 0.48 to 21.52 items L^-1, 35.76 to 3185.33 items kg^-1, and 0 to 2.73 items individual^-1 (0 to 168.48 items g^-1), respectively. The dominant shape and polymer of MPs in water and tadpole samples were polyester (PES) fibers, and polypropylene (PP) fibers and fragments were dominant in sediment samples. In addition, MPs were primarily <0.5 mm in length in all samples. Tadpole length was positively correlated to the number of MPs detected. The abundance, shape, and polymer distribution of MPs in tadpoles resembled that of water rather than sediment, suggesting that tadpoles likely take up MPs from the surrounding water. This study demonstrated that MPs are abundant in these small waterbodies and are ingested by resident tadpoles. This may suggest a pathway of MP entry into aquatic and terrestrial food webs.20:T400,The ingestion of plastics appears to be widespread throughout the animal kingdom with risks to individuals, ecosystems and human health. Despite growing information on the location, abundance and size distribution of plastics in the environment, it cannot be assumed that any given animal will ingest all sizes of plastic encountered. Here, we use published data to develop an allometric relationship between plastic consumption and animal size to estimate the size distribution of plastics feasibly ingested by animals. Based on more than 2000 gut content analyses from animals ranging over three orders of magnitude in size (lengths 9 mm to 10 m), body length alone accounts for 42% of the variance in the length of plastic an animal may ingest and indicates a size ratio of roughly 20:1 between animal body length and the largest plastic the animal may ingest. We expect this work to improve global assessments of plastic pollution risk by introducing a quantifiable link between animals and the plastics they can ingest.21:T981,

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