Culture of chestnut shoots from buds<i>in vitro</i>

Abstract

SummaryA method is described of growing chestnut shoots in vitro from lateral buds of 3-4 month-old seedlings cultured in a nutrient medium. The addition of BAP to the culture medium, optimally at a concentration of 1 mg l-1 caused the development of axillary shoots. Zeatin induced more vigorous shoots, but with little development of axillary shoots. Kinetin had no effect, compared to the control, without cytokinin. An adverse effect on growth was observed when GA3 was added to the cultures. Sub-cultures of single shoots and nodes excised from shoots on the initial cultures were established in a medium with 0.1 mg l-1 BAP to promote further shoot multiplication.