Effects of equine infectious anemia virus on hematopoietic progenitors in vitro

Abstract

Summary Direct effects of equine infectious anemia virus ( eiav ) on hematopoiesis in vitro were studied. Bone marrow mononuclear cells from clinically normal horses were incubated with 100 TCID 50 of ELAV/10 7 cells. These cells were cultured to assay for colonies derived from erythroid pro-genitors, granulocyte/monocyte progenitors, and fibroblastic progenitors. The eiav had a selective suppressive effect on the erythroid progenitors. Colony-forming units-erythroid were suppressed to 80% of that for medium controls ( P = 0.011). Burst-forming units-erythroid were suppressed to 70% of that for medium controls ( P = 0.003). Significant effect was not apparent on colony-forming units-granulocyte/macrophage or on colony-forming units-fibroblastic.