Strategies for high cell density cultivation of <i>Akkermansia muciniphila</i> and its potential metabolism

Abstract

Currently, there is significant interest in <i>Akkermansia muciniphila</i> as a promising next-generation probiotic, making it a hot topic in scientific research. However, to achieve efficient industrial production, there is an urgent need to develop an <i>in vitro</i> culture method to achieve high biomass using low-cost carbon sources such as glucose. This study aims to explore the high-density fermentation strategy of <i>A. muciniphila</i> by optimizing the culture process. This study also employs techniques such as LC-MS and RNA-Seq to explain the possible regulatory mechanism of high-density cell growth and increased cell surface hydrophobicity facilitating cell colonization of the gut <i>in vitro</i> culture. Overall, this research sheds light on the potential of <i>A. muciniphila</i> as a probiotic and provides valuable insights for future industrial production.