Phyto-mediated synthesis of zinc oxide nanoparticles from Clerodendrum infortunatum L. leaf extract and evaluation of antibacterial potential

Abstract

This scientific endeavor aims at green synthesis of zinc oxide nanoparticles (ZnONPs) using Clerodendrum infortunatum leaf aqueous extract. After synthesis they were characterized using different analytical techniques like UV–Vis spectroscopy, FTIR spectroscopy, X-ray diffraction analysis and SEM imaging. Furthermore, their antimicrobial activity via agar well diffusion method and minimum inhibitory concentration (MIC) analysis also shows the activity against Escherichia coli (MTCC 40), Staphylococcus aureus (MTCC 1114), and Klebsiella pneumoniae (MTCC 432). UV–Vis spectroscopy has shown the characteristic peak of ZnO at 356 nm. FTIR analysis revealed the role of phenols, proteins, carboxylic acid and its esters in fabricating ZnONPs. IR spectral peak at 671 cm−1 confirmed the presence of ZnO. The characterization result of SEM support that the synthesized nanoparticles are spherical in shape with 32 nm of size. Best inhibition was shown against E. coli with MIC value of 0.156 mg/ml. Overall, this work demonstrates the ecofriendly and cost-effective synthesis of ZnONPs along with good antibacterial activity compared with previous reports. C. infortunatum extract mediated zinc oxide nanoparticles (CIAQ-ZnONPs) can be utilized effectively for therapeutic and biomedical applications.