Culture-Delivery Live Probiotics Dressing for Accelerated Infected Wound Healing

Abstract

Probiotic therapy in infected wound healing is hindered by its low viability and colonization efficiency during treatments. Developing dressings that maintain metabolic activity and prevent the potential leakage of probiotics is imperative. Herein, a culture-delivery live probiotics hydrogel dressing is designed and synthesized, formed by gelatin modified with norbornene (GelNB) and sulfhydryl (GelSH), distributing <i>Lactobacillus reuteri</i> (<i>L. reuteri</i>)-laden alginate microspheres (AlgMPs). GelNB-GelSH hydrogel (GelNBSH) incorporating AlgMPs embedding <i>L. reuteri</i> (GelNBSH-L) possesses bioprintability and efficient polymerization that can maintain the activity of <i>L. reuteri</i> in situ, promote its proliferation, and limit its leakage. Thereby, GelNBSH-L achieved a sustainable antimicrobial effect against both <i>S. aureus</i> and <i>E. coli</i> (>90%). Above all, the results show that GelNBSH-L could ensure propitious viability and efficient antibacterial properties of probiotics, effectively inhibit the further development of bacterial infectious wounds and shorten the repair cycle, aiding in ameliorating future clinical probiotic biotherapy.