iScience · 2023 · 20 citations · 50 references
Life SciencesBiologyMolecular BiologyIntracellular TransportMolecular VirologyVirologyAntiviral TherapyNuclear Pore ComplexesNuclear TransportNuclear-cytoplasmic TransportProtein TraffickingViral EntryImportin-mediated Nuclear ImportImportin-mediated TransportNuclear Import PathwaysNuclear Pore Complex TransportNuclear Protein ImportProtein Nuclear ExportProtein Nuclear ImportRegulation Of Nuclear Import
Dengue virus (DENV) uses cellular nuclear transport machinery to import some proteins into the nucleus. Recently, the non-structural protein 3 (NS3) of DENV was localized in the nucleus of infected cells; however, its nuclear import mechanism is still unknown. In this study, we demonstrate that Ivermectin (IVM) inhibits the nuclear localization of NS3 through the inhibition of the Importin α/β1 pathway. We also report that Atorvastatin (ATV) can modulate the nuclear transport of NS3 protease and NS5 polymerase of DENV-2. On the other hand, we found that IVM and ATV treatments reduce the alteration of nuclear pore complex (NPC) proteins, and an IVM+ATV combination reduced DENV infection both in vitro and in vivo . Hence, we conclude that ATV transport inhibition is an additional antiviral effect of this drug, suggesting a potential anti-DENV therapy in combination with IVM.
50
The FDA-approved drug ivermectin inhibits the replication of SARS-CoV-2 in vitro
Leon Caly, Julian Druce, Mike Catton et al. · Antiviral Research · 2020 · 2.3K citations · Full text
Eloise Mastrangelo, Margherita Pezzullo, Tine De Burghgraeve et al. · Journal of Antimicrobial Chemotherapy · 2012 · 426 citations · Full text