Chemical Biology Approach to Reveal the Importance of Precise Subcellular Targeting for Intracellular <i>Staphylococcus aureus</i> Eradication

Abstract

Intracellular bacterial pathogens, such as <i>Staphylococcus aureus</i>, that may hide in intracellular vacuoles represent the most significant manifestation of bacterial persistence. They are critically associated with chronic infections and antibiotic resistance, as conventional antibiotics are ineffective against such intracellular persisters due to permeability issues and mechanistic reasons. Direct subcellular targeting of <i>S. aureus</i> vacuoles suggests an explicit opportunity for the eradication of these persisters, but a comprehensive understanding of the chemical biology nature and significance of precise <i>S. aureus</i> vacuole targeting remains limited. Here, we report an oligoguanidine-based peptidomimetic that effectively targets and eradicates intracellular <i>S. aureus</i> persisters in the phagolysosome lumen, and this oligomer was utilized to reveal the mechanistic insights linking precise targeting to intracellular antimicrobial efficacy. The oligomer has high cellular uptake via a receptor-mediated endocytosis pathway and colocalizes with <i>S. aureus</i> persisters in phagolysosomes as a result of endosome-lysosome interconversion and lysosome-phagosome fusion. Moreover, the observation of a bacterium's altered susceptibility to the oligomer following a modification in its intracellular localization offers direct evidence of the critical importance of precise intracellular targeting. In addition, eradication of intracellular <i>S. aureus</i> persisters was achieved by the oligomer's membrane/DNA dual-targeting mechanism of action; therefore, its effectiveness is not hampered by the hibernation state of the persisters. Such precise subcellular targeting of <i>S. aureus</i> vacuoles also increases the agent's biocompatibility by minimizing its interaction with other organelles, endowing excellent <i>in vivo</i> bacterial targeting and therapeutic efficacy in animal models.