Sanger Tree of Life RNA Extraction: Automated MagMax™ mirVana v1

Abstract

This protocol describes the automated extraction of RNA from multiple different tissue samples intended for RNA-Seq using the MagMax™ mirVana total RNA isolation kit and the Thermo Fisher KingFisher™ Apex. This process is highly effective for the majority of taxonomic groups covered by the Tree of Life Programme, however, challenging samples include corals, jellyfish and annelids. The output of this protocol is a highly concentrated RNA extract which can be diluted and submitted for RNA-Seq on Illumina NovaSeq.