High-Dose Vitamin B6 (Pyridoxine) Displays Strong Anti-Inflammatory Properties in Lipopolysaccharide-Stimulated Monocytes

Abstract

Vitamin B6 is shown to have anti-inflammatory properties, which makes it an interesting nutraceutical agent. Vitamin B6 deficiency is well established as a contributor to inflammatory-related conditions, whilst B6 supplementation can reverse these inflammatory effects. There is less information available regarding the effects of high-dose vitamin B6 supplementation as a therapeutic agent. This study set out to examine the effects of high-dose vitamin B6 on an LPS-stimulated monocyte/macrophage cell population via an analysis of protein and gene expression using an RT2 profiler PCR array for Human Innate and Adaptive Immune responses. It was identified that high-dose vitamin B6 has a global anti-inflammatory effect on lipopolysaccharide-induced inflammation in monocyte/macrophage cells by downregulating the key broad-spectrum inflammatory mediators <i>CCL2</i>, <i>CCL5</i>, <i>CXCL2</i>, <i>CXCL8</i>, <i>CXCL10</i>, <i>CCR4</i>, <i>CCR5</i>, <i>CXCR3</i>, <i>IL-1β</i>, <i>IL-5</i>, <i>IL-6</i>, <i>IL-10</i>, <i>IL-18</i>, <i>IL-23-a</i>, <i>TNF-α</i>, <i>CSF2</i>, <i>DDX58</i>, <i>NLRP3</i>, <i>NOD1</i>, <i>NOD2</i>, <i>TLR-1 -2 -4 -5 -7 -8 -9</i>, <i>MYD88</i>, <i>C3</i>, <i>FOXP3</i>, <i>STAT1</i>, <i>STAT3</i>, <i>STAT6</i>, <i>LYZ</i>, <i>CASP-1</i>, <i>CD4</i>, <i>HLA-E</i>, <i>MAPK1</i>, <i>MAPK8 MPO</i>, <i>MX-1</i>, <i>NF-κβ</i>, <i>NF-κβ1A</i>, <i>CD14</i>, <i>CD40</i>, <i>CD40LG</i>, <i>CD86</i>, <i>Ly96</i>, <i>ICAM1</i>, <i>IRF3</i>, <i>ITGAM</i>, and <i>IFCAM2</i>. The outcomes of this study show promise regarding vitamin B6 within the context of a potent broad-spectrum anti-inflammatory mediator and could prove useful as an adjunct treatment for inflammatory-related diseases.