Design of Water–Soluble Rotaxane-Capped Superparamagnetic, Ultrasmall Fe₃O₄ Nanoparticles for Targeted NIR Fluorescence Imaging in Combination with Magnetic Resonance Imaging

Abstract

Integrating an NIR fluorescent probe with a magnetic resonance imaging (MRI) agent to harvest complementary imaging information is challenging. Here, we have designed water-soluble, biocompatible, noncytotoxic, bright-NIR-emitting, sugar-functionalized, mechanically interlocked molecules (MIMs)-capped superparamagnetic ultrasmall Fe 3 O 4 NPs for targeted multimodal imaging. Dual-functional stoppers containing an unsymmetrical NIR squaraine dye interlocked within a macrocycle to construct multifunctional MIMs are developed with enhanced NIR fluorescence efficiency and durability. One of the stoppers of the axle is composed of a lipophilic cationic TPP + functionality to target mitochondria, and the other stopper comprises a dopamine-containing catechol group to anchor at the surface of the synthesized Fe 3 O 4 NPs. Fe 3 O 4 NPs surface-coated with targeted NIR rotaxanes help to deliver ultrasmall magnetic NPs specifically inside the mitochondria. Two carbohydrate moieties are conjugated with the macrocycle of the rotaxane via click chemistry to improve the water solubility of MitoSQRot-(Carb-OH) 2 -DOPA-Fe 3 O 4 NPs. Water-soluble, rotaxane-capped Fe 3 O 4 NPs are used for live-cell mitochondria-targeted NIR fluorescence confocal imaging, 3D and multicolor imaging in combination with T 2 -weighted MRI on a 9.4 T MR scanner with a high relaxation rate ( r 2 ) of 180.7 mM –1 s –1 . Biocompatible, noncytotoxic, ultrabright NIR rotaxane-capped superparamagnetic ultrasmall monodisperse Fe 3 O 4 NPs could be a promising agent for targeted multimodal imaging applications.