Soil phosphorus availability alters the correlations between root phosphorus‐uptake rates and net photosynthesis of dominant C₃ and C₄ species in a typical temperate grassland of Northern China

Abstract

Summary Phosphorus (P) fertilization can alleviate a soil P deficiency in grassland ecosystems. Understanding plant functional traits that enhance P uptake can improve grassland management. We measured impacts of P addition on soil chemical and microbial properties, net photosynthetic rate ( P n ) and nonstructural carbohydrate concentrations ([NSC]), and root P‐uptake rate (PUR), morphology, anatomy, and exudation of two dominant grass species: Leymus chinensis (C 3 ) and Cleistogenes squarrosa (C 4 ). For L. chinensis , PUR and P n showed a nonlinear correlation. Growing more adventitious roots compensated for the decrease in P transport per unit root length, so that it maintained a high PUR. For C. squarrosa , PUR and P n presented a linear correlation. Increased P n was associated with modifications in root morphology, which further enhanced its PUR and a greater surplus of photosynthate and significantly stimulated root exudation (proxied by leaf [Mn]), which had a greater impact on rhizosheath micro‐environment and microbial PLFAs. Our results present correlations between the PUR and the P n of L. chinensis and C. squarrosa and reveal that NSC appeared to drive the modifications of root morphology and exudation; they provide more objective basis for more efficient P‐input in grasslands to address the urgent problem of P deficiency.