Science · 2023 · 93 citations · 28 references
Life SciencesEndocrinologyPhysiologyCell SignalingIntegrative PhysiologyMucosal ImmunologyAntimicrobial PeptidesAppetiteGastrointestinal FunctionGut HealthGut MicrobiomeGut MicrobiotaAntibacterial PeptidesAntimicrobial Peptide RegulationDigestive Tract PeptidesGut Hormone RegulationHost Antimicrobial PeptidesMucosal Antimicrobial PeptidesPeptide Yy PhysiologyPeptide Yy Signaling
The mammalian gut secretes a family of multifunctional peptides that affect appetite, intestinal secretions, and motility whereas others regulate the microbiota. We have found that peptide YY (PYY 1-36 ), but not endocrine PYY 3-36 , acts as an antimicrobial peptide (AMP) expressed by gut epithelial paneth cells (PC). PC-PYY is packaged into secretory granules and is secreted into and retained by surface mucus, which optimizes PC-PYY activity. Although PC-PYY shows some antibacterial activity, it displays selective antifungal activity against virulent Candida albicans hyphae—but not the yeast form. PC-PYY is a cationic molecule that interacts with the anionic surfaces of fungal hyphae to cause membrane disruption and transcriptional reprogramming that selects for the yeast phenotype. Hence, PC-PYY is an antifungal AMP that contributes to the maintenance of gut fungal commensalism.
28
NIH Image to ImageJ: 25 years of image analysis
Caroline A Schneider, Wayne Rasband, Kevin W. Eliceiri · Nature Methods · 2012 · 65.2K citations
DADA2: High-resolution sample inference from Illumina amplicon data
Benjamin J. Callahan, Paul J. McMurdie, Michael Rosen et al. · Nature Methods · 2016 · 36.3K citations