Vitamin E Ameliorates Impaired Ovarian Development, Oxidative Stress, and Disrupted Lipid Metabolism in Oreochromis niloticus Fed with a Diet Containing Olive Oil Instead of Fish Oil

Abstract

Aquaculture feed containing olive oil (OO) instead of fish oil (FO) can cause oxidative stress and impair gonad development in fish. We determined the effect of dietary OO-induced oxidative stress on ovarian development, and explored whether vitamin E (VE) could mitigate negative effects. Female Nile tilapia (<i>Oreochromis niloticus</i>) were fed for 10 weeks with four diets: 5% OO + 70 mg/kg VE, 5% OO + 200 mg/kg VE, 5% FO + 70 mg/kg VE, or 5% FO + 200 mg/kg VE. Dietary OO reduced the specific growth rate and gonadosomatic index, inhibited superoxide dismutase and catalase, delayed ovarian development, decreased serum sex hormone levels, and reduced ovarian triglyceride and n-3 highly unsaturated fatty acid contents. The transcript levels of genes encoding sex hormone receptors (<i>erα</i>, <i>fshr</i>, <i>lhr</i>) and components of the lipid metabolism pathway (<i>pparα</i>, <i>pparγ</i>, <i>hsl</i>, <i>accα</i>, <i>elovl6</i>), the <i>nrf2</i> signaling pathway (<i>nrf2</i>, <i>keap1</i>), and the <i>nf-κb</i> signaling pathway (<i>nf-κb</i>, <i>tnfα</i>, <i>infγ</i>, <i>il1β</i>) differed between the 70VE/OO and 70VE/FO groups. Supplementation with 200 mg/kg VE mitigated the adverse effects of OO by improving antioxidant capacity and alleviating inflammation and abnormal lipid metabolism. This may be because VE is an antioxidant and it can regulate the <i>nrf2</i>-<i>nf-κb</i> signaling pathway.