Angewandte Chemie International Edition · 2023 · 20 citations · 39 references
Life SciencesImmunologyMolecular BiologyMolecular DiagnosticsMucosal ImmunologyNucleic Acid BiochemistryAirway DiseasesEosinophilic DisordersEosinophil BiomarkersEosinophilic Airway DiseaseIn Vitro SelectionLateral Flow Assay DesignLateral Flow AssaysNucleasesSelectivitySubstrate Affinity
The first protein-binding allosteric RNA-cleaving DNAzyme (RCD) obtained by direct in vitro selection against eosinophil peroxidase (EPX), a validated marker for airway eosinophilia, is described. The RCD has nanomolar affinity for EPX, shows high selectivity against related peroxidases and other eosinophil proteins, and is resistant to degradation by mammalian nucleases. An optimized RCD was used to develop both fluorescence and lateral flow assays, which were evaluated using 38 minimally processed patient sputum samples (23 non-eosinophilic, 15 eosinophilic), producing a clinical sensitivity of 100 % and specificity of 96 %. This RCD-based lateral flow assay should allow for rapid evaluation of airway eosinophilia as an aid for guiding asthma therapy.
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Asthma exacerbations and sputum eosinophil counts: a randomised controlled trial
Ruth H. Green, Christopher E. Brightling, Susan McKenna et al. · The Lancet · 2002 · 1.8K citations
Induced Sputum Cell Counts in Healthy Adults
J. Belda, Richard Leigh, Krishnan Parameswaran et al. · American Journal of Respiratory and Critical Care Medicine · 2000 · 391 citations